Serveur d'exploration sur la glutarédoxine

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SA-inducible Arabidopsis glutaredoxin interacts with TGA factors and suppresses JA-responsive PDF1.2 transcription.

Identifieur interne : 000C58 ( Main/Exploration ); précédent : 000C57; suivant : 000C59

SA-inducible Arabidopsis glutaredoxin interacts with TGA factors and suppresses JA-responsive PDF1.2 transcription.

Auteurs : Ivan Ndamukong [Allemagne] ; Ayed Al Abdallat ; Corinna Thurow ; Benjamin Fode ; Mark Zander ; Ralf Weigel ; Christiane Gatz

Source :

RBID : pubmed:17397508

Descripteurs français

English descriptors

Abstract

Salicylic acid (SA) is a plant signaling molecule that mediates the induction of defense responses upon attack by a variety of pathogens. Moreover, it antagonizes gene induction by the stress signaling molecule jasmonic acid (JA). Several SA-responsive genes are regulated by basic/leucine zipper-type transcription factors of the TGA family. TGA factors interact with NPR1, a central regulator of many SA-induced defense responses including SA/JA antagonism. In order to identify further regulatory proteins of SA-dependent signaling pathways, a yeast protein interaction screen with tobacco TGA2.2 as bait and an Arabidopsis thaliana cDNA prey library was performed and led to the identification of a member of the glutaredoxin family (GRX480, encoded by At1g28480). Glutaredoxins are candidates for mediating redox regulation of proteins because of their capacity to catalyze disulfide transitions. This agrees with previous findings that the redox state of both TGA1 and NPR1 changes under inducing conditions. Transgenic Arabidopsis plants ectopically expressing GRX480 show near wild-type expression of standard marker genes for SA- and xenobiotic-inducible responses. In contrast, transcription of the JA-dependent defensin gene PDF1.2 was antagonized by transgenic GRX480. This, together with the observation that GRX480 transcription is SA-inducible and requires NPR1, suggests a role of GRX480 in SA/JA cross-talk. Suppression of PDF1.2 by GRX480 depends on the presence of TGA factors, indicating that the GRX480/TGA interaction is effective in planta.

DOI: 10.1111/j.1365-313X.2007.03039.x
PubMed: 17397508


Affiliations:


Links toward previous steps (curation, corpus...)


Le document en format XML

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<term>Amino Acid Sequence (MeSH)</term>
<term>Arabidopsis (drug effects)</term>
<term>Arabidopsis (genetics)</term>
<term>Arabidopsis (metabolism)</term>
<term>Arabidopsis Proteins (genetics)</term>
<term>Arabidopsis Proteins (metabolism)</term>
<term>Base Sequence (MeSH)</term>
<term>Basic-Leucine Zipper Transcription Factors (genetics)</term>
<term>Basic-Leucine Zipper Transcription Factors (metabolism)</term>
<term>Blotting, Northern (MeSH)</term>
<term>Chromatin Immunoprecipitation (MeSH)</term>
<term>Cyclopentanes (pharmacology)</term>
<term>Gene Expression Regulation, Plant (drug effects)</term>
<term>Glutaredoxins (MeSH)</term>
<term>Models, Biological (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Nuclear Proteins (genetics)</term>
<term>Nuclear Proteins (metabolism)</term>
<term>Oxidoreductases (genetics)</term>
<term>Oxidoreductases (metabolism)</term>
<term>Oxylipins (MeSH)</term>
<term>Plants, Genetically Modified (MeSH)</term>
<term>Protein Binding (drug effects)</term>
<term>Salicylic Acid (pharmacology)</term>
<term>Transcription, Genetic (drug effects)</term>
<term>Transcriptional Activation (MeSH)</term>
<term>Two-Hybrid System Techniques (MeSH)</term>
<term>Xenobiotics (pharmacology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr">
<term>Acide salicylique (pharmacologie)</term>
<term>Activation de la transcription (MeSH)</term>
<term>Arabidopsis (effets des médicaments et des substances chimiques)</term>
<term>Arabidopsis (génétique)</term>
<term>Arabidopsis (métabolisme)</term>
<term>Cyclopentanes (pharmacologie)</term>
<term>Données de séquences moléculaires (MeSH)</term>
<term>Facteurs de transcription à motif basique et à glissière à leucines (génétique)</term>
<term>Facteurs de transcription à motif basique et à glissière à leucines (métabolisme)</term>
<term>Glutarédoxines (MeSH)</term>
<term>Immunoprécipitation de la chromatine (MeSH)</term>
<term>Liaison aux protéines (effets des médicaments et des substances chimiques)</term>
<term>Modèles biologiques (MeSH)</term>
<term>Oxidoreductases (génétique)</term>
<term>Oxidoreductases (métabolisme)</term>
<term>Oxylipines (MeSH)</term>
<term>Protéines d'Arabidopsis (génétique)</term>
<term>Protéines d'Arabidopsis (métabolisme)</term>
<term>Protéines nucléaires (génétique)</term>
<term>Protéines nucléaires (métabolisme)</term>
<term>Régulation de l'expression des gènes végétaux (effets des médicaments et des substances chimiques)</term>
<term>Séquence d'acides aminés (MeSH)</term>
<term>Séquence nucléotidique (MeSH)</term>
<term>Technique de Northern (MeSH)</term>
<term>Techniques de double hybride (MeSH)</term>
<term>Transcription génétique (effets des médicaments et des substances chimiques)</term>
<term>Végétaux génétiquement modifiés (MeSH)</term>
<term>Xénobiotique (pharmacologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en">
<term>Arabidopsis Proteins</term>
<term>Basic-Leucine Zipper Transcription Factors</term>
<term>Nuclear Proteins</term>
<term>Oxidoreductases</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en">
<term>Arabidopsis</term>
<term>Gene Expression Regulation, Plant</term>
<term>Protein Binding</term>
<term>Transcription, Genetic</term>
</keywords>
<keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr">
<term>Arabidopsis</term>
<term>Liaison aux protéines</term>
<term>Régulation de l'expression des gènes végétaux</term>
<term>Transcription génétique</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en">
<term>Arabidopsis</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr">
<term>Arabidopsis</term>
<term>Facteurs de transcription à motif basique et à glissière à leucines</term>
<term>Oxidoreductases</term>
<term>Protéines d'Arabidopsis</term>
<term>Protéines nucléaires</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en">
<term>Arabidopsis</term>
<term>Arabidopsis Proteins</term>
<term>Basic-Leucine Zipper Transcription Factors</term>
<term>Nuclear Proteins</term>
<term>Oxidoreductases</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr">
<term>Arabidopsis</term>
<term>Facteurs de transcription à motif basique et à glissière à leucines</term>
<term>Oxidoreductases</term>
<term>Protéines d'Arabidopsis</term>
<term>Protéines nucléaires</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr">
<term>Acide salicylique</term>
<term>Cyclopentanes</term>
<term>Xénobiotique</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en">
<term>Cyclopentanes</term>
<term>Salicylic Acid</term>
<term>Xenobiotics</term>
</keywords>
<keywords scheme="MESH" xml:lang="en">
<term>Amino Acid Sequence</term>
<term>Base Sequence</term>
<term>Blotting, Northern</term>
<term>Chromatin Immunoprecipitation</term>
<term>Glutaredoxins</term>
<term>Models, Biological</term>
<term>Molecular Sequence Data</term>
<term>Oxylipins</term>
<term>Plants, Genetically Modified</term>
<term>Transcriptional Activation</term>
<term>Two-Hybrid System Techniques</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr">
<term>Activation de la transcription</term>
<term>Données de séquences moléculaires</term>
<term>Glutarédoxines</term>
<term>Immunoprécipitation de la chromatine</term>
<term>Modèles biologiques</term>
<term>Oxylipines</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
<term>Technique de Northern</term>
<term>Techniques de double hybride</term>
<term>Végétaux génétiquement modifiés</term>
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<div type="abstract" xml:lang="en">Salicylic acid (SA) is a plant signaling molecule that mediates the induction of defense responses upon attack by a variety of pathogens. Moreover, it antagonizes gene induction by the stress signaling molecule jasmonic acid (JA). Several SA-responsive genes are regulated by basic/leucine zipper-type transcription factors of the TGA family. TGA factors interact with NPR1, a central regulator of many SA-induced defense responses including SA/JA antagonism. In order to identify further regulatory proteins of SA-dependent signaling pathways, a yeast protein interaction screen with tobacco TGA2.2 as bait and an Arabidopsis thaliana cDNA prey library was performed and led to the identification of a member of the glutaredoxin family (GRX480, encoded by At1g28480). Glutaredoxins are candidates for mediating redox regulation of proteins because of their capacity to catalyze disulfide transitions. This agrees with previous findings that the redox state of both TGA1 and NPR1 changes under inducing conditions. Transgenic Arabidopsis plants ectopically expressing GRX480 show near wild-type expression of standard marker genes for SA- and xenobiotic-inducible responses. In contrast, transcription of the JA-dependent defensin gene PDF1.2 was antagonized by transgenic GRX480. This, together with the observation that GRX480 transcription is SA-inducible and requires NPR1, suggests a role of GRX480 in SA/JA cross-talk. Suppression of PDF1.2 by GRX480 depends on the presence of TGA factors, indicating that the GRX480/TGA interaction is effective in planta.</div>
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<AbstractText>Salicylic acid (SA) is a plant signaling molecule that mediates the induction of defense responses upon attack by a variety of pathogens. Moreover, it antagonizes gene induction by the stress signaling molecule jasmonic acid (JA). Several SA-responsive genes are regulated by basic/leucine zipper-type transcription factors of the TGA family. TGA factors interact with NPR1, a central regulator of many SA-induced defense responses including SA/JA antagonism. In order to identify further regulatory proteins of SA-dependent signaling pathways, a yeast protein interaction screen with tobacco TGA2.2 as bait and an Arabidopsis thaliana cDNA prey library was performed and led to the identification of a member of the glutaredoxin family (GRX480, encoded by At1g28480). Glutaredoxins are candidates for mediating redox regulation of proteins because of their capacity to catalyze disulfide transitions. This agrees with previous findings that the redox state of both TGA1 and NPR1 changes under inducing conditions. Transgenic Arabidopsis plants ectopically expressing GRX480 show near wild-type expression of standard marker genes for SA- and xenobiotic-inducible responses. In contrast, transcription of the JA-dependent defensin gene PDF1.2 was antagonized by transgenic GRX480. This, together with the observation that GRX480 transcription is SA-inducible and requires NPR1, suggests a role of GRX480 in SA/JA cross-talk. Suppression of PDF1.2 by GRX480 depends on the presence of TGA factors, indicating that the GRX480/TGA interaction is effective in planta.</AbstractText>
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<name sortKey="Ndamukong, Ivan" sort="Ndamukong, Ivan" uniqKey="Ndamukong I" first="Ivan" last="Ndamukong">Ivan Ndamukong</name>
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